NKIRAS1 antibody (N-Term)

Catalog No. ABIN2786171

The Rabbit Polyclonal anti-NKIRAS1 antibody has been validated for WB. It is suitable to detect NKIRAS1 in samples from Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for NKIRAS1 antibody (N-Term) (ABIN2786171)

Target: NKIRAS1 (NFKB Inhibitor Interacting Ras-Like 1 (NKIRAS1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NKIRAS1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NKIRAS1 Antibody

Binding Specificity: N-Term

Sequence: CETMEDVYMA SVETDRGVKE QLHLYDTRGL QEGVELPKHY FSFADGFVLV

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against NKIRAS1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NKIRAS1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 192 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NKIRAS1

Target: NKIRAS1 (NFKB Inhibitor Interacting Ras-Like 1 (NKIRAS1))

Alternative Name: NKIRAS1

Background: NKIRAS1 is an atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. NKIRAS1 may act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.
Alias Symbols: KBRAS1, kappaB-Ras1
Protein Interaction Partner: XRCC2, UBC, APP, NFKBIB, SMURF2, BMPR1B, TGFBR1, NFKBIA, ACVR1,
Protein Size: 192

Molecular Weight: 22 kDa

Gene ID: 28512

NCBI Accession: NM_020345, NP_065078

UniProt: Q9NYS0