EHD4 antibody (Middle Region)

Catalog No. ABIN2786207

Cited in 1 publication. The Rabbit Polyclonal anti-EHD4 antibody (ABIN2786207) specifically detects EHD4 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig and Rabbit samples.

Quick Overview for EHD4 antibody (Middle Region) (ABIN2786207)

Target: EHD4 (EH-Domain Containing 4 (EHD4))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EHD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EHD4 Antibody

Binding Specificity: Middle Region

Purpose: EHD4 Antibody - middle region

Sequence: LMNLISQEET STPTQLVQGG AFDGTTEGPF NQGYGEGAKE GADEEEWVVA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against EHD4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EHD4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP55433-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-EHD4 antibody (ABIN2786207)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for EHD4

Target: EHD4 (EH-Domain Containing 4 (EHD4))

Alternative Name: EHD4

Background:

Background Information: EHD4 is involved in the control of trafficking at the early endosome and regulates exit of cargo toward both the recycling compartment and the late endocytic pathway.

Gene Name: EH-domain containing 4

Alternative Symbols: PAST4

Protein Name: EH domain-containing protein 4

Molecular Weight: 61kDa

Gene ID: 30844

NCBI Accession: NP_644670

UniProt: Q9H223