NAPEPLD antibody (N-Term)

Catalog No. ABIN2786441

Cited in 1 publication. The Rabbit Polyclonal anti-NAPEPLD antibody (ABIN2786441) specifically detects NAPEPLD in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Horse and Dog samples.

Quick Overview for NAPEPLD antibody (N-Term) (ABIN2786441)

Target: NAPEPLD (N-Acyl Phosphatidylethanolamine phospholipase D (NAPEPLD))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Horse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAPEPLD antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NAPEPLD Antibody

Binding Specificity: N-Term

Purpose: NAPE-PLD Antibody - N-terminal region

Sequence: TWKNPSIPNV LRWLIMEKDH SSVPSSKEEL DKELPVLKPY FITNPEEAGV

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NAPE-PLD. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NAPE-PLD

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP55927-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-NAPEPLD antibody (ABIN2786441)

Sousa-Valente, Varga, Torres-Perez, Jenes, Wahba, Mackie, Cravatt, Ueda, Tsuboi, Santha, Jancso, Tailor, Avelino, Nagy et al.: "Inflammation of peripheral tissues and injury to peripheral nerves induce differing effects in the expression of the calcium-sensitive N-arachydonoylethanolamine-synthesizing enzyme and related ..." in: The Journal of comparative neurology, Vol. 525, Issue 8, pp. 1778-1796, (2016) (PubMed).

Target Details for NAPEPLD

Target: NAPEPLD (N-Acyl Phosphatidylethanolamine phospholipase D (NAPEPLD))

Alternative Name: NAPE-PLD

Background:

Background Information: NAPE-PLD hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. NAPE-PLD is responsible for the generation of anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors

Gene Name: N-acyl phosphatidylethanolamine phospholipase D

Alternative Symbols: FMP30, C7orf18, NAPE-PLD

Protein Name: N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D

Molecular Weight: 46 kDa

Gene ID: 222236

NCBI Accession: NP_945341

UniProt: Q6IQ20