MLH1 antibody (N-Term)

Catalog No. ABIN2786501

This Rabbit Polyclonal antibody specifically detects MLH1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit and Saccharomyces cerevisiae.

Quick Overview for MLH1 antibody (N-Term) (ABIN2786501)

Target: MLH1 (MutL Homolog 1 (MLH1))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MLH1 Antibody

Binding Specificity: N-Term

Sequence: MIENCLDAKS TSIQVIVKEG GLKLIQIQDN GTGIRKEDLD IVCERFTTSK

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 83%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against MLH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MLH1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 756 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: MLH1

Background: This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC.This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: COCA2, FCC2, HNPCC, HNPCC2, MGC5172, hMLH1
Protein Interaction Partner: SPERT, CXorf57, PSMA1, MYOG, MSH3, KPNA2, MLH3, ZC3H11A, EXO1, PMS2, MMS19, PCNA, DES, CTSB, ATF2, CKB, CAPN5, ASS1, TRIM23, ARAF, APRT, ANXA6, ANXA2, ALDOA, ACTG2, ACTG1, TXN2, GABARAP, EXOC3, CKAP4, AGR2, ZER1, NDRG1, AIFM1, SELENBP1, SQSTM1, FCGBP, ABC
Protein Size: 756

Molecular Weight: 84 kDa

Gene ID: 4292

NCBI Accession: NM_000249, NP_000240

UniProt: P40692

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response