BLMH antibody (Middle Region)

Catalog No. ABIN2786515

The Rabbit Polyclonal anti-BLMH antibody (ABIN2786515) specifically detects BLMH in WB. The antibody is reactive with Human, Rat, Mouse, Dog, Guinea Pig, Zebrafish (Danio rerio), Cow, Horse and Rabbit samples.

Quick Overview for BLMH antibody (Middle Region) (ABIN2786515)

Target: BLMH (Bleomycin Hydrolase (BLMH))

Reactivity: Human, Rat, Mouse, Dog, Guinea Pig, Zebrafish (Danio rerio), Cow, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BLMH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BLMH Antibody

Binding Specificity: Middle Region

Purpose: BLMH Antibody - middle region

Sequence: EYLSNMVGGR KTLYNNQPID FLKKMVAASI KDGEAVWFGC DVGKHFNSKL

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against BLMH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human BLMH

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56106-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for BLMH

Target: BLMH (Bleomycin Hydrolase (BLMH))

Alternative Name: BLMH

Background:

Background Information: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution, however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Bleomycin hydrolase

Alternative Symbols: BH, BMH

Protein Name: Bleomycin hydrolase

Molecular Weight: 52kDa

Gene ID: 642

NCBI Accession: NP_000377

UniProt: Q13867