RPL5 antibody (N-Term)

Catalog No. ABIN2786525

The Rabbit Polyclonal anti-RPL5 antibody is suitable to detect RPL5 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit and Cow. It has been validated for WB.

Quick Overview for RPL5 antibody (N-Term) (ABIN2786525)

Target: RPL5 (Ribosomal Protein L5 (RPL5))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RPL5 Antibody

Binding Specificity: N-Term

Purpose: RPL5 Antibody - N-terminal region

Sequence: RLVIQDKNKY NTPKYRMIVR VTNRDIICQI AYARIEGDMI VCAAYAHELP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against RPL5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RPL5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56125-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RPL5

Target: RPL5 (Ribosomal Protein L5 (RPL5))

Alternative Name: RPL5

Background:

Background Information: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18P family of ribosomal proteins and component of the 60S subunit. The encoded protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The encoded protein may also function to inhibit tumorigenesis through the activation of downstream tumor suppressors and the downregulation of oncoprotein expression. Mutations in this gene have been identified in patients with Diamond-Blackfan Anemia (DBA). This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome.

Gene Name: ribosomal protein L5

Alternative Symbols: L5, uL18, MSTP030, PPP1R135

Protein Name: 60S ribosomal protein L5

Molecular Weight: 34kDa

Gene ID: 6125

NCBI Accession: NP_000960

UniProt: P46777