MTRF1L antibody (N-Term)

Catalog No. ABIN2786636

This Rabbit Polyclonal antibody specifically detects MTRF1L in WB. It exhibits reactivity toward Human.

Quick Overview for MTRF1L antibody (N-Term) (ABIN2786636)

Target: MTRF1L (Mitochondrial Translational Release Factor 1-Like (MTRF1L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTRF1L antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MTRF1L Antibody

Binding Specificity: N-Term

Purpose: MTRF1L Antibody - N-terminal region

Sequence: MRSRVLWGAA RWLWPRRAVG PARRPLSSGS PPLEELFTRG GPLRTFLERQ

Predicted Reactivity: Cow: 86%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 77%, Rabbit: 92%, Rat: 83%

Characteristics: This is a rabbit polyclonal antibody against MTRF1L. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RF1ML

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56319-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MTRF1L

Target: MTRF1L (Mitochondrial Translational Release Factor 1-Like (MTRF1L))

Alternative Name: MTRF1L

Background:

Background Information: The protein encoded by this gene plays a role in mitochondrial translation termination, and is thought to be a release factor that is involved in the dissociation of the complete protein from the final tRNA, the ribosome, and the cognate mRNA. This protein acts upon UAA and UAG stop codons, but has no in vitro activity against UGA, which encodes tryptophan in human mitochondrion, or, the mitochondrial non-cognate stop codons, AGA and AGG. This protein shares sequence similarity to bacterial release factors. Pseudogenes of this gene are found on chromosomes 4, 8, and 11. Alternative splicing results in multiple transcript variants.

Gene Name: mitochondrial translational release factor 1 like

Alternative Symbols: MRF1L, HMRF1L, mtRF1a

Protein Name: peptide chain release factor 1-like, mitochondrial

Molecular Weight: 29 kDa

Gene ID: 54516

NCBI Accession: NP_001107656

UniProt: Q9UGC7