ATP5F1D antibody (C-Term)

Catalog No. ABIN2786638

The Rabbit Polyclonal anti-ATP5F1D antibody is suitable to detect ATP5F1D in samples from Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig and Horse. It has been validated for WB.

Quick Overview for ATP5F1D antibody (C-Term) (ABIN2786638)

Target: ATP5F1D (ATP synthase subunit delta, mitochondrial (ATP5F1D))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5F1D antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP5F1D Antibody

Binding Specificity: C-Term

Purpose: Atp5d Antibody - C-terminal region

Sequence: SVQLLAEEAV TLDMLDLGAA RANLEKAQSE LSGAADEAAR AEIQIRIEAN

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rat: 93%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against Atp5d. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56323-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATP5F1D

Target: ATP5F1D (ATP synthase subunit delta, mitochondrial (ATP5F1D))

Alternative Name: Atp5d

Background:

Targeted Subunit: delta, mitochondrial

Background Information: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Gene Name: ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit

Alternative Symbols: C85518, Atp5f1d, AA960090, AI876556, AU020773, 0610008F14Rik, 1500000I11Rik

Protein Name: ATP synthase subunit delta, mitochondrial

Molecular Weight: 18kDa

Gene ID: 66043

NCBI Accession: NP_079589

UniProt: Q9D3D9

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process