PSMB1 antibody (Middle Region)

Catalog No. ABIN2786714

This Rabbit Polyclonal antibody specifically detects PSMB1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Saccharomyces cerevisiae, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig and Horse.It has been mentioned in 1 publication

Quick Overview for PSMB1 antibody (Middle Region) (ABIN2786714)

Target: PSMB1 (Proteasome (Prosome, Macropain) Subunit, beta Type, 1 (PSMB1))

Reactivity: Human, Mouse, Rat, Rabbit, Saccharomyces cerevisiae, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PSMB1 Antibody

Binding Specificity: Middle Region

Sequence: NNKAMTTGAI AAMLSTILYS RRFFPYYVYN IIGGLDEEGK GAVYSFDPVG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against PSMB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PSMB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 241 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PSMB1 antibody (ABIN2786714)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for PSMB1

Target: PSMB1 (Proteasome (Prosome, Macropain) Subunit, beta Type, 1 (PSMB1))

Alternative Name: PSMB1

Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB1 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species.The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: FLJ25321, HC5, KIAA1838, PMSB1, PSC5
Protein Interaction Partner: MAPRE3, UBC, TRAF1, TRIM27, KRT15, KRT13, KIFC3, CDKN1B, PSMA7, HAUS2, PSMD14, PSEN1, PSMG3, PSMB8, PSMB7, PSMB6, PSMB5, PSMB4, PSMB3, PSMB2, PSMA6, PSMA5, PSMA4, PSMA3, PSMA1, HNRNPA2B1, DDB2, SMEK1, AP3M1, PSME4, RPP38, RPP30, SEC13, PYCRL, HECW2, PSMA2
Protein Size: 241

Molecular Weight: 26 kDa

Gene ID: 5689

NCBI Accession: NM_002793, NP_002784

UniProt: P20618

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA