PSMB4 antibody (Middle Region)

Catalog No. ABIN2786719

The Rabbit Polyclonal anti-PSMB4 antibody is suitable to detect PSMB4 in samples from Human, Mouse, Rat, Cow, Rabbit, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio) and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for PSMB4 antibody (Middle Region) (ABIN2786719)

Target: PSMB4 (Proteasome (Prosome, Macropain) Subunit, beta Type, 4 (PSMB4))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMB4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PSMB4 Antibody

Binding Specificity: Middle Region

Purpose: PSMB4 Antibody - middle region

Sequence: SRRSKMNPLW NTMVIGGYAD GESFLGYVDM LGVAYEAPSL ATGYGAYLAQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 83%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against PSMB4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PSMB4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56466-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PSMB4

Target: PSMB4 (Proteasome (Prosome, Macropain) Subunit, beta Type, 4 (PSMB4))

Alternative Name: PSMB4

Background:

Targeted Subunit: beta type-4

Background Information: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB4 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit.The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Proteasome (prosome, macropain) subunit, beta type, 4

Alternative Symbols: HN3, HsN3, PRAAS3, PROS26, PROS-26

Protein Name: Proteasome subunit beta type-4

Molecular Weight: 29kDa

Gene ID: 5692

NCBI Accession: NP_002787

UniProt: P28070

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA