PSMD10 antibody (N-Term)

Catalog No. ABIN2786728

This Rabbit Polyclonal antibody specifically detects PSMD10 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) and Cow.

Quick Overview for PSMD10 antibody (N-Term) (ABIN2786728)

Target: PSMD10 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 10 (PSMD10))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMD10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PSMD10 Antibody

Binding Specificity: N-Term

Sequence: KERILADKSL ATRTDQDSRT ALHWACSAGH TEIVEFLLQL GVPVNDKDDA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against Psmd10. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 231 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PSMD10

Target: PSMD10 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 10 (PSMD10))

Alternative Name: Psmd10

Background: Psmd10 acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly,Psmd10 is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module By similarity.Psmd10 acts as an oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression of this gene is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Psmd10 regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4.
Alias Symbols: AW554874
Protein Interaction Partner: Psmc4, Rb1, Cebpa, Cdk4,
Protein Size: 231

Molecular Weight: 25 kDa

Gene ID: 53380

NCBI Accession: NM_016883, NP_058579

UniProt: Q9Z2X2

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Ubiquitin Proteasome Pathway