PSME2 antibody (Middle Region)

Catalog No. ABIN2786731

The Rabbit Polyclonal anti-PSME2 antibody is suitable to detect PSME2 in samples from Human, Mouse, Rat, Dog, Horse, Zebrafish (Danio rerio), Cow, Guinea Pig, Rabbit and Sheep. It has been validated for WB.

Quick Overview for PSME2 antibody (Middle Region) (ABIN2786731)

Target: PSME2 (Proteasome (Prosome, Macropain) Activator Subunit 2 (PA28 Beta) (PSME2))

Reactivity: Human, Mouse, Rat, Dog, Horse, Zebrafish (Danio rerio), Cow, Guinea Pig, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSME2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PSME2 Antibody

Binding Specificity: Middle Region

Purpose: PSME2 Antibody - middle region

Sequence: SKETHVMDYR ALVHERDEAA YGELRAMVLD LRAFYAELYH IISSNLEKIV

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against PSME2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PSME2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56486-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PSME2

Target: PSME2 (Proteasome (Prosome, Macropain) Activator Subunit 2 (PA28 Beta) (PSME2))

Alternative Name: PSME2

Background:

Targeted Subunit: 2

Background Information: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13.

Gene Name: Proteasome (prosome, macropain) activator subunit 2 (PA28 beta)

Alternative Symbols: PA28B, REGbeta, PA28beta

Protein Name: Proteasome activator complex subunit 2

Molecular Weight: 27kDa

Gene ID: 5721

NCBI Accession: NP_002809

UniProt: Q9UL46

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA