PKC eta antibody (N-Term)

Catalog No. ABIN2786852

This Rabbit Polyclonal antibody specifically detects PKC eta in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Guinea Pig, Dog, Horse, Zebrafish (Danio rerio) and Pig.

Quick Overview for PKC eta antibody (N-Term) (ABIN2786852)

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Dog, Horse, Zebrafish (Danio rerio), Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC eta antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PKC eta Antibody

Binding Specificity: N-Term

Purpose: PRKCH Antibody - N-terminal region

Sequence: KKGHQLLDPY LTVSVDQVRV GQTSTKQKTN KPTYNEEFCA NVTDGGHLEL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against PRKCH. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRKCH

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56703-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PKC eta

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Alternative Name: PRKCH

Background:

Background Information: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.

Gene Name: Protein kinase C, eta

Alternative Symbols: PKCL, PKC-L, PRKCL, nPKC-eta

Protein Name: Protein kinase C eta type

Molecular Weight: 78 kDa

Gene ID: 5583

NCBI Accession: NP_006246

UniProt: P24723

Pathways: Myometrial Relaxation and Contraction, Thromboxane A2 Receptor Signaling