MED31 antibody (N-Term)

Catalog No. ABIN2786919

The Rabbit Polyclonal anti-MED31 antibody has been validated for WB and ChIP. It is suitable to detect MED31 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for MED31 antibody (N-Term) (ABIN2786919)

Target: MED31 (Mediator Complex Subunit 31 (MED31))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED31 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-MED31 Antibody

Binding Specificity: N-Term

Sequence: MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MED31. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MED31

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 131 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED31

Target: MED31 (Mediator Complex Subunit 31 (MED31))

Alternative Name: MED31

Background: MED31 is the component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal
Alias Symbols: 3110004H13Rik, CGI-125, FLJ27436, FLJ36714, Soh1
Protein Interaction Partner: UBC, CDK19, CDK8, MED19, MED26, FBXW7, GADD45G, FAM118B, DAZAP2, SELENBP1, TK1, SMN1, NR4A1, CDKN1A, ANXA7, HTT, MED28, MED18, MED12, QKI, TADA2A, ZSCAN1, MED7, HGH1, ASCC2, ZBTB45, RBM23, MED9, EEF1G, PMF1, HNRNPUL1, MOB4, ORAI2, HSPB1, CACNB4, ERH, C2or
Protein Size: 131

Molecular Weight: 16 kDa

Gene ID: 51003

NCBI Accession: NM_016055, NP_057144

UniProt: Q9Y3C7

Pathways: Regulation of Lipid Metabolism by PPARalpha