C11orf73 antibody (Middle Region)

Catalog No. ABIN2786966

The Rabbit Polyclonal anti-C11orf73 antibody (ABIN2786966) specifically detects C11orf73 in WB. The antibody is reactive with Human, Mouse, Rat, Horse, Cow, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Pig and Saccharomyces cerevisiae samples.

Quick Overview for C11orf73 antibody (Middle Region) (ABIN2786966)

Target: C11orf73 (Chromosome 11 Open Reading Frame 73 (C11orf73))

Reactivity: Human, Mouse, Rat, Horse, Cow, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C11orf73 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-C11orf73 Antibody

Binding Specificity: Middle Region

Purpose: HIKESHI Antibody - middle region

Sequence: DNFYNFASSF AVSQAQMTPS PSEMFIPANV VLKWYENFQR RLAQNPLFWK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 90%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Yeast: 90%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against C11orf73. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human C11orf73

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP56903-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for C11orf73

Target: C11orf73 (Chromosome 11 Open Reading Frame 73 (C11orf73))

Alternative Name: HIKESHI

Background:

Background Information: This gene encodes an evolutionarily conserved nuclear transport receptor that mediates heat-shock-induced nuclear import of 70 kDa heat-shock proteins (Hsp70s) through interactions with FG-nucleoporins. The protein mediates transport of the ATP form but not the ADP form of Hsp70 proteins under conditions of heat shock stress. Structural analyses demonstrate that the protein forms an asymmetric homodimer and that the N-terminal domain consists of a jelly-roll/beta-sandwich fold structure that contains hydrophobic pockets involved in FG-nucleoporin recognition. Reduction of RNA expression levels in HeLa cells using small interfering RNAs results in inhibition of heat shock-induced nuclear accumulation of Hsp70s, indicating a role for this gene in regulation of Hsp70 nuclear import during heat shock stress.

Gene Name: Hikeshi, heat shock protein nuclear import factor

Alternative Symbols: HLD13, L7RN6, OPI10, HSPC138, HSPC179, C11orf73

Protein Name: protein Hikeshi

Molecular Weight: 21kDa

Gene ID: 51501

NCBI Accession: NP_057485

UniProt: Q53FT3

Pathways: Protein targeting to Nucleus