OTUB1 antibody (Middle Region)

Catalog No. ABIN2787016

This anti-OTUB1 antibody is a Rabbit Polyclonal antibody detecting OTUB1 in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Zebrafish (Danio rerio).

Quick Overview for OTUB1 antibody (Middle Region) (ABIN2787016)

Target: OTUB1 (OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OTUB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-OTUB1 Antibody

Binding Specificity: Middle Region

Sequence: KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against OTUB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human OTUB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 271 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for OTUB1

Target: OTUB1 (OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1))

Alternative Name: OTUB1

Background: The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitin
Alias Symbols: FLJ20113, FLJ40710, HSPC263, MGC111158, MGC4584, OTB1, OTU1
Protein Interaction Partner: UBE2D4, ERN1, UBE2D3, UBC, SUMO2, TRAF3, NEDD8, SUMO1, NMRAL1, TP53, UBE2D1, RNF2, KDM1A, UBE2N, SMAD4, SMAD3, SMAD2, TNFSF12, BIRC3, BIRC2, SMAD9, ESR1, ACTB, UBE2D2, RNF126, ECT2, OTUD6B, DSTN, RFFL, TRIM63, TRIM55, PJA1, USP28, TRIM54, USP21, USP15, UB
Protein Size: 271

Molecular Weight: 31 kDa

Gene ID: 55611

NCBI Accession: NM_017670, NP_060140

UniProt: Q96FW1