CHCHD3 antibody (N-Term)

Catalog No. ABIN2787033

This Rabbit Polyclonal antibody specifically detects CHCHD3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Horse, Rabbit, Sheep, Guinea Pig and Cow. It has been mentioned in 2+ publications

Quick Overview for CHCHD3 antibody (N-Term) (ABIN2787033)

Target: CHCHD3 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 3 (CHCHD3))

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Sheep, Guinea Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHCHD3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHCHD3 Antibody

Binding Specificity: N-Term

Purpose: CHCHD3 Antibody - N-terminal region

Sequence: RMKESSPSGS KSQRYSGAYG ASVSDEELKR RVAEELALEQ AKKESEDQKR

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against CHCHD3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CHCHD3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP57039-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CHCHD3 antibody (ABIN2787033)

Ott, Ross, Straub, Thiede, Götz, Goosmann, Krischke, Mueller, Krohne, Rudel, Kozjak-Pavlovic: "Sam50 functions in mitochondrial intermembrane space bridging and biogenesis of respiratory complexes." in: Molecular and cellular biology, Vol. 32, Issue 6, pp. 1173-88, (2012) (PubMed).

Darshi, Trinh, Murphy, Taylor: "Targeting and import mechanism of coiled-coil helix coiled-coil helix domain-containing protein 3 (ChChd3) into the mitochondrial intermembrane space." in: The Journal of biological chemistry, Vol. 287, Issue 47, pp. 39480-91, (2012) (PubMed).

Target Details for CHCHD3

Target: CHCHD3 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 3 (CHCHD3))

Alternative Name: CHCHD3

Background:

Background Information: The function of this protein remains unknown.

Gene Name: Coiled-coil-helix-coiled-coil-helix domain containing 3

Alternative Symbols: Mic19, MINOS3, MICOS19, PPP1R22

Protein Name: Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial

Molecular Weight: 26kDa

Gene ID: 54927

NCBI Accession: NP_060282

UniProt: Q9NX63