Nodal antibody (Middle Region)

Catalog No. ABIN2787062

This Rabbit Polyclonal antibody specifically detects Nodal in WB. It exhibits reactivity toward Human, Rat, Mouse, Dog, Horse, Cow, Guinea Pig, Rabbit and Zebrafish (Danio rerio).

Quick Overview for Nodal antibody (Middle Region) (ABIN2787062)

Target: Nodal (NODAL) (Nodal Homolog (NODAL))

Reactivity: Human, Rat, Mouse, Dog, Horse, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nodal antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nodal Antibody

Binding Specificity: Middle Region

Sequence: EFHPTNHAYI QSLLKRYQPH RVPSTCCAPV KTKPLSMLYV DNGRVLLDHH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against NODAL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NODAL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 347 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Nodal

Target: Nodal (NODAL) (Nodal Homolog (NODAL))

Alternative Name: NODAL

Background: The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development.
Alias Symbols: MGC138230, HTX5
Protein Size: 347

Molecular Weight: 37 kDa

Gene ID: 4838

NCBI Accession: NM_018055, NP_060525

UniProt: Q96S42

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of Endopeptidase Activity