Nodal antibody (Center)

Catalog No. ABIN2856412

Product Details anti-Nodal Antibody

Target: Nodal (NODAL) (Nodal Homolog (NODAL))

Binding Specificity: Center

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nodal antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Cow (Bovine)

Cross-Reactivity (Details): Bovine (93 %)

Characteristics: Rabbit Polyclonal antibody to Nodal (nodal homolog (mouse))
Nodal antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Nodal. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ELISA 1:1000-1:10000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceELISA1:1000-1:10000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: Human ESC

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Nodal

Target: Nodal (NODAL) (Nodal Homolog (NODAL))

Alternative Name: Nodal (NODAL Products)

Synonyms: NODAL antibody, Tg.413d antibody, HTX5 antibody, nodal4 antibody, nodal4-A antibody, nr4 antibody, xnr4 antibody, Xnr-1 antibody, nodal antibody, nr1 antibody, nr1-A antibody, nodal growth differentiation factor antibody, nodal homolog (mouse) antibody, nodal antibody, nodal growth differentiation factor L homeolog antibody, nodal homolog 1 L homeolog antibody, Nodal antibody, NODAL antibody, nodal.L antibody, nodal1.L antibody

Background: The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development.

Cellular Localization: Secreted

Molecular Weight: 40 kDa

Gene ID: 4838

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of Endopeptidase Activity