TDP1 antibody (Middle Region)

Catalog No. ABIN2787112

Product Details anti-TDP1 Antibody

Target: TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Horse, Cow, Guinea Pig, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDP1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ETNVYLIGST PGRFQGSHRD NWGHFRLRKL LQAHAPSTPK GECWPIVGQF

Predicted Reactivity: Cow: 92%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Tdp1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 579 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TDP1

Target: TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))

Alternative Name: Tdp1 (TDP1 Products)

Background: Tdp1 is a DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. It catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. It hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. It acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. It has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. It has no exonuclease activity towards DNA or RNA with a 3'phosphate.
Alias Symbols: 2810481F14Rik, 4921509N21Rik, AI838772, AW493413, E430034L06Rik, SCAN1, MLZ-501
Protein Size: 579

Molecular Weight: 64 kDa

Gene ID: 104884

NCBI Accession: NM_028354, NP_082630