SMEK1 antibody (Middle Region)

Catalog No. ABIN2787340

This Rabbit Polyclonal antibody specifically detects SMEK1 in WB. It exhibits reactivity toward Mouse, Human, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for SMEK1 antibody (Middle Region) (ABIN2787340)

Target: SMEK1 (Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1))

Reactivity: Mouse, Human, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMEK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMEK1 Antibody

Binding Specificity: Middle Region

Sequence: YWKALEDVDY VQTFKGLKLR FEQQRERQDN PKLDSMRSIL RNHRYRRDAR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SMEK1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SMEK1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 820 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMEK1

Target: SMEK1 (Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1))

Alternative Name: SMEK1

Background: SMEK1 is the regulatory subunit of serine/threonine-protein phosphatase 4.SMEK1 may regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.
Alias Symbols: FLFL1, KIAA2010, MSTP033, smk-1, smk1, PP4R3A
Protein Interaction Partner: HUWE1, UBC, PPP4R2, BRCC3, SF3A1, HNRNPR, TELO2, MTA2, PSMB1, PSMA2, DDB2, CAST, ARCN1, AMPD2, ACACA, CASK, POLR2A, IGBP1, PPP4C, HDAC1, MAPK14,
Protein Size: 820

Molecular Weight: 94 kDa

Gene ID: 55671

NCBI Accession: NM_032560, NP_115949

Pathways: Regulation of Carbohydrate Metabolic Process