Glutaredoxin 2 antibody (Middle Region)

Catalog No. ABIN2787395

The Rabbit Polyclonal anti-Glutaredoxin 2 antibody (ABIN2787395) specifically detects Glutaredoxin 2 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Sheep samples.

Quick Overview for Glutaredoxin 2 antibody (Middle Region) (ABIN2787395)

Target: Glutaredoxin 2 (GRX2)

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutaredoxin 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Glutaredoxin 2 Antibody

Binding Specificity: Middle Region

Purpose: GLRX2 Antibody - middle region

Sequence: NVNYKVVELD LLEYGNQFQD ALYKMTGERT VPRIFVNGTF IGGATDTHRL H

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 93%, Rabbit: 86%, Rat: 86%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against GLRX2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GLRX2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP57792-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Glutaredoxin 2

Target: Glutaredoxin 2 (GRX2)

Alternative Name: GLRX2

Background:

Background Information: GLRX2 is a glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress.GLRX2 is involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress.GLRX2 acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides.GLRX2 can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions.GLRX2 efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression of GLRX2 decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.

Gene Name: Glutaredoxin 2

Alternative Symbols: GRX2, CGI-133

Protein Name: Glutaredoxin-2, mitochondrial

Molecular Weight: 18kDa

Gene ID: 51022

NCBI Accession: NP_932066

UniProt: Q9NS18

Pathways: Cell RedoxHomeostasis