MED16 antibody (Middle Region)

Catalog No. ABIN2787461

Product Details anti-MED16 Antibody

Target: MED16 (Mediator Complex Subunit 16 (MED16))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED16 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: IVALSWLHNG VKLALHVEKS GASSFGEKFS RVKFSPSLTL FGGKPMEGWI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against MED16. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human MED16

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED16

Target: MED16 (Mediator Complex Subunit 16 (MED16))

Alternative Name: MED16 (MED16 Products)

Synonyms: THRAP5 antibody, MED16 antibody, DRIP92 antibody, TRAP95 antibody, 95kDa antibody, A630083L04 antibody, Thrap5 antibody, Trap95 antibody, mediator complex subunit 16 antibody, mediator complex subunit 16 L homeolog antibody, MED16 antibody, Med16 antibody, med16.L antibody

Background: MED16 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Protein Interaction Partner: HECW2, CDK19, CDK8, UBC, MED19, MED26, EPAS1, FBXW7, MED11, MED8, MED29, MED9, MED18, MED4, RALY, MED13, MED12, MED24, MED21, MED14, MED1, CTDP1, HIST1H3A, MED10, MED25, MED15, VDR, TRA, MED28, OBFC1, ZC3H13, QKI, TADA2A, SUPT7L, SUPT3H, MYC, KAT2A, TRRAP
Protein Size: 663

Molecular Weight: 72 kDa

Gene ID: 10025

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha