CEBPA antibody (Middle Region)

Catalog No. ABIN2787465

The Rabbit Polyclonal anti-CEBPA antibody has been validated for WB and IHC. It is suitable to detect CEBPA in samples from Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Dog, Rabbit, Horse and Sheep. There are 2+ publications available.

Quick Overview for CEBPA antibody (Middle Region) (ABIN2787465)

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Guinea Pig, Dog, Rabbit, Horse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CEBPA Antibody

Binding Specificity: Middle Region

Sequence: RRERNNIAVR KSRDKAKQRN VETQQKVLEL TSDNDRLRKR VEQLSRELDT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 79%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 79%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against CEBPA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CEBPA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 358 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CEBPA antibody (ABIN2787465)

Miglino, Roth, Tamm, Borger: "House dust mite extract downregulates C/EBP? in asthmatic bronchial smooth muscle cells." in: The European respiratory journal, Vol. 38, Issue 1, pp. 50-8, (2011) (PubMed).

Borger, Miglino, Baraket, Black, Tamm, Roth: "Impaired translation of CCAAT/enhancer binding protein alpha mRNA in bronchial smooth muscle cells of asthmatic patients." in: The Journal of allergy and clinical immunology, Vol. 123, Issue 3, pp. 639-45, (2009) (PubMed).

Target Details for CEBPA

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Alternative Name: CEBPA

Background: CEBPA is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-2385 U34070.1 592-2976
Alias Symbols: C/EBP-alpha, CEBP
Protein Interaction Partner: CEBPB, ZBTB16, RARA, HDAC1, BHLHE41, UBC, CEBPE, BATF2, BATF3, YY1AP1, ATF5, BATF, FOSL1, FOS, DBP, CEBPG, CEBPA, ATF4, ATF3, UBE3A, SKP2, RASGRF1, C1QBP, CDKN1A, SRA1, CDK4, PIAS1, Fbxw7, GRIN3A, RAB34, NAA16, POLR1E, MRTO4, AK5, MACF1, KAT5, AKAP9, SMC1
Protein Size: 358

Molecular Weight: 37 kDa

Gene ID: 1050

NCBI Accession: NM_004364, NP_004355

UniProt: P49715

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation