UBE2K antibody (N-Term)

Catalog No. ABIN2787510

The Rabbit Polyclonal anti-UBE2K antibody has been validated for WB. It is suitable to detect UBE2K in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Goat, Horse, Rabbit, Guinea Pig and Saccharomyces cerevisiae. There is 1 publication available.

Quick Overview for UBE2K antibody (N-Term) (ABIN2787510)

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Goat, Horse, Rabbit, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2K antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBE2K Antibody

Binding Specificity: N-Term

Sequence: FTELRGEIAG PPDTPYEGGR YQLEIKIPET YPFNPPKVRF ITKIWHPNIS

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against UBE2K. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2K

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 200 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-UBE2K antibody (ABIN2787510)

Fromm-Dornieden, von der Heyde, Lytovchenko, Salinas-Riester, Brenig, Beissbarth, Baumgartner: "Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells." in: BMC molecular biology, Vol. 13, pp. 9, (2012) (PubMed).

Target Details for UBE2K

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Alternative Name: UBE2K

Background: UBE2K belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation.The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene.
Alias Symbols: DKFZp564C1216, DKFZp686J24237, E2-25K, HIP2, HYPG, LIG, UBC1
Protein Interaction Partner: DDI1, DTX3, MIPOL1, TRIM39, ANAPC11, RNF138, SIAH1, RNF5, REL, FZR1, UBC, UBA1, MARCH7, AMFR, TP53, MDM2, BAG3, PRRG4, IQCB1, HTT, CDC6, WDR1, TYMS, TXN, TXNDC17, WDR61, ITCH, NEDD4L, WWP2, NXF1, NUB1, PIAS4, CASP12, SUMO1, SUMO2, DIABLO, UBE2I, CCNB1, BR
Protein Size: 200

Molecular Weight: 22 kDa

Gene ID: 3093

NCBI Accession: NM_005339, NP_005330

UniProt: P61086