NFE2 antibody (Middle Region)

Catalog No. ABIN2787523

The Rabbit Polyclonal anti-NFE2 antibody has been validated for WB. It is suitable to detect NFE2 in samples from Human, Mouse, Rat, Horse, Cow, Guinea Pig, Dog and Rabbit.

Quick Overview for NFE2 antibody (Middle Region) (ABIN2787523)

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Reactivity: Human, Mouse, Rat, Horse, Cow, Guinea Pig, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFE2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NFE2 Antibody

Binding Specificity: Middle Region

Sequence: KPLSLSGLLS EPLQDPLALL DIGLPAGPPK PQEDPESDSG LSLNYSDAES

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against NFE2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFE2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 373 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFE2

Target: NFE2 (Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2))

Alternative Name: NFE2

Background: NFE2 is required for activity at the locus control region (LCR) upstream of the globin gene complexes.It requires p18 NF-E2 for binding to the NF-E2 motif. NFE2 may play a role in all aspects of hemoglobin production: globin synthesis, heme synthesis, and the procurement of iron.
Alias Symbols: NF-E2, p45
Protein Interaction Partner: BTRC, NFE2L3, NFE2L2, NFE2, ATF4, Dlg4, TXN, ITCH, Yap1, Nedd4, WWP1, MAPK8, KAT2B, SCAND1, SSX5, HMGN1, ELF5, PHYHIP, MAFF, MAFG, PEX14, MAFK, CREBBP,
Protein Size: 373

Molecular Weight: 41 kDa

Gene ID: 4778

NCBI Accession: NM_006163, NP_006154

UniProt: Q16621