GCFC2 antibody (N-Term)

Catalog No. ABIN2787542

The Rabbit Polyclonal anti-GCFC2 antibody is suitable to detect GCFC2 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Cow, Horse and Rabbit. It has been validated for WB.

Quick Overview for GCFC2 antibody (N-Term) (ABIN2787542)

Target: GCFC2 (GC-Rich Sequence DNA-Binding Factor 2 (GCFC2))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Cow, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCFC2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GCFC2 Antibody

Binding Specificity: N-Term

Purpose: C2orf3 Antibody - N-terminal region

Sequence: SEPDDHEKRI PFTLRPQTLR QRMAEESISR NEETSEESQE DEKQDTWEQQ

Predicted Reactivity: Cow: 79%, Dog: 100%, Guinea Pig: 92%, Horse: 85%, Human: 100%, Mouse: 85%, Rabbit: 83%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against C2orf3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human C2orf3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58090-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GCFC2

Target: GCFC2 (GC-Rich Sequence DNA-Binding Factor 2 (GCFC2))

Alternative Name: C2orf3

Background:

Background Information: The first mRNA transcript isolated for C2orf3 gene was part of an artificial chimera derived from two distinct gene transcripts and a primer used in the cloning process (see Genbank accession M29204). C2orf3 belongs to the GCF family. It is a factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters.The first mRNA transcript isolated for this gene was part of an artificial chimera derived from two distinct gene transcripts and a primer used in the cloning process (see Genbank accession M29204). A positively charged amino terminus present only in the chimera was determined to bind GC-rich DNA, thus mistakenly thought to identify a transcription factor gene.

Gene Name: GC-rich sequence DNA-binding factor 2

Alternative Symbols: GCF, TCF9, DNABF, C2orf3

Protein Name: GC-rich sequence DNA-binding factor 2

Molecular Weight: 89kDa

Gene ID: 6936

NCBI Accession: NP_003194

UniProt: P16383