TRIM25 antibody (Middle Region)

Catalog No. ABIN2787550

The Rabbit Polyclonal anti-TRIM25 antibody (ABIN2787550) specifically detects TRIM25 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Rabbit and Horse samples.

Quick Overview for TRIM25 antibody (Middle Region) (ABIN2787550)

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM25 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TRIM25 Antibody

Binding Specificity: Middle Region

Purpose: TRIM25 Antibody - middle region

Sequence: LLDASETTST RKIKEEEKRV NSKFDTIYQI LLKKKSEIQT LKEEIEQSLT

Predicted Reactivity: Cow: 79%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against TRIM25. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRIM25

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58105-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for TRIM25

Target: TRIM25 (Tripartite Motif Containing 25 (TRIM25))

Alternative Name: TRIM25

Background:

Background Information: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Gene Name: Tripartite motif containing 25

Alternative Symbols: EFP, Z147, RNF147, ZNF147

Protein Name: E3 ubiquitin/ISG15 ligase TRIM25

Molecular Weight: 71kDa

Gene ID: 7706

NCBI Accession: NP_005073

UniProt: Q14258