MED14 antibody (Middle Region)

Catalog No. ABIN2787568

The Rabbit Polyclonal anti-MED14 antibody (ABIN2787568) specifically detects MED14 in WB and ChIP. The antibody is reactive with Human, Mouse and Rabbit samples.

Quick Overview for MED14 antibody (Middle Region) (ABIN2787568)

Target: MED14 (Mediator Complex Subunit 14 (MED14))

Reactivity: Human, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED14 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-MED14 Antibody

Binding Specificity: Middle Region

Purpose: MED14 Antibody - middle region

Sequence: AADREDSPAM ALLLQQFKEN IQDLVFRTKT GKQTRTNAKR KLSDDPCPVE

Predicted Reactivity: Human: 100%, Mouse: 79%, Rabbit: 86%

Characteristics: This is a rabbit polyclonal antibody against MED14. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MED14

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58139-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED14

Target: MED14 (Mediator Complex Subunit 14 (MED14))

Alternative Name: MED14

Background:

Targeted Subunit: 14

Background Information: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.

Gene Name: Mediator complex subunit 14

Alternative Symbols: CSRP, RGR1, CRSP2, EXLM1, CXorf4, CRSP150, DRIP150, TRAP170

Protein Name: Mediator of RNA polymerase II transcription subunit 14

Molecular Weight: 161 kDa

Gene ID: 9282

NCBI Accession: NP_004220

UniProt: O60244

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha