HMGB2 antibody (Middle Region)

Catalog No. ABIN2787574

The Rabbit Polyclonal anti-HMGB2 antibody is suitable to detect HMGB2 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio) and Pig. It has been validated for WB and IHC.

Quick Overview for HMGB2 antibody (Middle Region) (ABIN2787574)

Target: HMGB2 (High Mobility Group Box 2 (HMGB2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HMGB2 Antibody

Binding Specificity: Middle Region

Purpose: HMGB2 Antibody - middle region

Sequence: DREMKNYVPP KGDKKGKKKD PNAPKRPPSA FFLFCSEHRP KIKSEHPGLS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 87%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 80%

Characteristics: This is a rabbit polyclonal antibody against HMGB2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HMGB2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58157-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HMGB2

Target: HMGB2 (High Mobility Group Box 2 (HMGB2))

Alternative Name: HMGB2

Background:

Background Information: HMGB2 is a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. HMGB2 was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: High mobility group box 2

Alternative Symbols: HMG2

Protein Name: High mobility group protein B2

Molecular Weight: 24kDa

Gene ID: 3148

NCBI Accession: NP_002120

UniProt: P26583

Pathways: Cellular Response to Molecule of Bacterial Origin