KDM4B antibody (Middle Region)

Catalog No. ABIN2787592

The Rabbit Polyclonal anti-KDM4B antibody (ABIN2787592) specifically detects KDM4B in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Horse, Pig, Cow and Guinea Pig samples.

Quick Overview for KDM4B antibody (Middle Region) (ABIN2787592)

Target: KDM4B (Lysine (K)-Specific Demethylase 4B (KDM4B))

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM4B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KDM4B Antibody

Binding Specificity: Middle Region

Purpose: JMJD2B Antibody - middle region

Sequence: SASRASLKAK LLRRSHRKRS QPKKPKPEDP KFPGEGTAGA ALLEEAGGSV

Predicted Reactivity: Cow: 92%, Dog: 93%, Guinea Pig: 83%, Horse: 93%, Human: 100%, Mouse: 82%, Pig: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against JMJD2B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human JMJD2B

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58207-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for KDM4B

Target: KDM4B (Lysine (K)-Specific Demethylase 4B (KDM4B))

Alternative Name: JMJD2B

Background:

Background Information: JMJD2 family proteins are classified into one group with JD2H and TUDOR domains and another group without JD2H or TUDOR domains. Because JMJD2C gene (also known as GASC1 gene) is amplified in esophageal squamous cell carcinoma (ESCC), JMJD2 family genes are cancer-associated genes. Human genes corresponding to KIAA0677, KIAA0876, KIAA0780 and FLJ10251 cDNAs were designated JMJD2A, JMJD2B, JMJD2C, and JMJD2D, respectively. In addition, JMJD2D homologous genes within human genome sequences AP002383.3 and AP001264.4 were designated JMJD2E and JMJD2F, respectively. C2HC2HC2- and C5HC2-type Cys (His) clusters were identified as the region conserved among JMJD2A (1064 aa), JMJD2B (1096 aa), and JMJD2C (1056 aa) proteins. JMJD2A, JMJD2B and JMJD2C consist of JmjN, JmjC, JD2H, and two TUDOR domains.

Gene Name: Lysine (K)-specific demethylase 4B

Alternative Symbols: JMJD2B, TDRD14B

Protein Name: Lysine-specific demethylase 4B

Molecular Weight: 122kDa

Gene ID: 23030

NCBI Accession: NP_055830

UniProt: O94953

Pathways: Warburg Effect