H2AFY antibody (Middle Region)

Catalog No. ABIN2787622

The Rabbit Polyclonal anti-H2AFY antibody (ABIN2787622) specifically detects H2AFY in WB. The antibody is reactive with Human, Rat, Mouse, Cow, Horse, Dog, Guinea Pig, Rabbit and Pig samples.

Quick Overview for H2AFY antibody (Middle Region) (ABIN2787622)

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Reactivity: Human, Rat, Mouse, Cow, Horse, Dog, Guinea Pig, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFY antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-H2AFY Antibody

Binding Specificity: Middle Region

Purpose: H2AFY Antibody - middle region

Sequence: PVSKKAGGKK GARKSKKQGE VSKAASADST TEGTPADGFT VLSTKSLFLG

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against H2AFY. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human H2AFY

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58283-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for H2AFY

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Alternative Name: H2AFY

Background:

Background Information: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. H2AFY is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Name: H2A histone family, member Y

Alternative Symbols: H2A.y, H2A/y, H2AFY, mH2A1, H2AF12M, MACROH2A1.1, macroH2A1.2

Protein Name: Core histone macro-H2A.1

Molecular Weight: 39kDa

Gene ID: 9555

NCBI Accession: NP_001035248

UniProt: O75367