HIST1H1E antibody (N-Term)

Catalog No. ABIN2787625

The Rabbit Polyclonal anti-HIST1H1E antibody (ABIN2787625) specifically detects HIST1H1E in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Cow and Guinea Pig samples.

Quick Overview for HIST1H1E antibody (N-Term) (ABIN2787625)

Target: HIST1H1E (Histone H1.4 (HIST1H1E))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H1E antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HIST1H1E Antibody

Binding Specificity: N-Term

Purpose: HIST1H1E Antibody - N-terminal region

Sequence: MSETAPAAPA APAPAEKTPV KKKARKSAGA AKRKASGPPV SELITKAVAA

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against HIST1H1E. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HIST1H1E

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58288-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HIST1H1E

Target: HIST1H1E (Histone H1.4 (HIST1H1E))

Alternative Name: HIST1H1E

Background:

Background Information: Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Gene Name: Histone cluster 1, H1e

Alternative Symbols: H1E, H1.4, H1F4, RMNS, H1s-4, HIST1H1E, dJ221C16.5

Protein Name: Histone H1.4

Molecular Weight: 22 kDa

Gene ID: 3008

NCBI Accession: NP_005312

UniProt: P10412