ACTN1 antibody (N-Term)

Catalog No. ABIN2787669

The Rabbit Polyclonal anti-ACTN1 antibody (ABIN2787669) specifically detects ACTN1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Zebrafish (Danio rerio) and Horse samples.

Quick Overview for ACTN1 antibody (N-Term) (ABIN2787669)

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Zebrafish (Danio rerio), Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ACTN1 Antibody

Binding Specificity: N-Term

Purpose: ACTN1 Antibody - N-terminal region

Sequence: DHYDSQQTND YMQPEEDWDR DLLLDPAWEK QQRKTFTAWC NSHLRKAGTQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 75%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against ACTN1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACTN1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58413-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ACTN1

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Alternative Name: ACTN1

Background:

Background Information: Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Actinin, alpha 1

Alternative Symbols: BDPLT15

Protein Name: Alpha-actinin-1

Molecular Weight: 103kDa

Gene ID: 87

NCBI Accession: NP_001093

UniProt: P12814

Pathways: Cell-Cell Junction Organization