COL1A2 antibody (Middle Region)

Catalog No. ABIN2787684

This Rabbit Polyclonal antibody specifically detects COL1A2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Cow, Goat, Guinea Pig, Horse and Zebrafish (Danio rerio).

Quick Overview for COL1A2 antibody (Middle Region) (ABIN2787684)

Target: COL1A2 (Collagen, Type I, alpha 2 (COL1A2))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Goat, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL1A2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-COL1A2 Antibody

Binding Specificity: Middle Region

Sequence: PGSVGPAGPR GPAGPSGPAG KDGRTGHPGT VGPAGIRGPQ GHQGPAGPPG

Predicted Reactivity: Cow: 100%, Dog: 93%, Goat: 79%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against COL1A2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human COL1A2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1366 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for COL1A2

Target: COL1A2 (Collagen, Type I, alpha 2 (COL1A2))

Alternative Name: COL1A2

Background: COL1A2 is the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity.This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: OI4
Protein Interaction Partner: UBQLN1, SGTA, PDGFB, PDGFA, COL1A1, PAN2, LIG4, MYOC, SHBG, UBC, MMP9, COL5A3, CD44, APP, P4HB, ITGA2B, TGFBI, LUM, BGN, VWF, ITGA2, SPARC, CD36, ITGB3, FN1, DCN, CD93,
Protein Size: 1366

Molecular Weight: 150 kDa

Gene ID: 1278

NCBI Accession: NM_000089, NP_000080

UniProt: P08123