GPX4 antibody (Middle Region)

Catalog No. ABIN2787700

The Rabbit Polyclonal anti-GPX4 antibody (ABIN2787700) specifically detects GPX4 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Zebrafish (Danio rerio), Guinea Pig, Cow, Goat and Saccharomyces cerevisiae samples.

Quick Overview for GPX4 antibody (Middle Region) (ABIN2787700)

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Guinea Pig, Cow, Goat, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GPX4 Antibody

Binding Specificity: Middle Region

Purpose: GPX4 Antibody - middle region

Sequence: QUGKTEVNYT QLVDLHARYA ECGLRILAFP CNQFGKQEPG SNEEIKEFAA

Predicted Reactivity: Cow: 86%, Goat: 86%, Guinea Pig: 86%, Human: 100%, Mouse: 86%, Rat: 86%, Yeast: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against GPX4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GPX4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58473-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Alternative Name: GPX4

Background:

Background Information: Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.

Gene Name: Glutathione peroxidase 4

Alternative Symbols: MCSP, SMDS, GPx-4, PHGPx, snGPx, GSHPx-4, snPHGPx

Protein Name: Phospholipid hydroperoxide glutathione peroxidase, mitochondrial

Molecular Weight: 19 kDa

Gene ID: 2879

NCBI Accession: NP_002076

UniProt: P36969