PEF1 antibody (Middle Region)

Catalog No. ABIN2787715

Product Details anti-PEF1 Antibody

Target: PEF1 (Penta-EF-Hand Domain Containing 1 (PEF1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEF1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: WKFIQQWKNL FQQYDRDRSG SISYTELQQA LSQMGYNLSP QFTQLLVSRY

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 93%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against PEF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PEF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 284 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PEF1

Target: PEF1 (Penta-EF-Hand Domain Containing 1 (PEF1))

Alternative Name: PEF1 (PEF1 Products)

Synonyms: 2600002E23Rik antibody, Peflin antibody, ABP32 antibody, PEF1A antibody, zgc:100787 antibody, penta-EF hand domain containing 1 antibody, penta-EF-hand domain containing 1 antibody, penta-EF-hand domain containing 1 L homeolog antibody, Pef1 antibody, PEF1 antibody, pef1 antibody, pef1.L antibody

Background: PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1, MIM 114170), sorcin (SRI, MIM 182520), grancalcin (GCA, MIM 607030), and ALG2.PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1, MIM 114170), sorcin (SRI, MIM 182520), grancalcin (GCA, MIM 607030), and ALG2 (PDCD6, MIM 601057) (Kitaura et al., 2001 [PubMed 11278427]).[supplied by OMIM]. Sequence Note: removed 1 base from the 3' end that did not align to the reference genome assembly. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1640 AB026628.1 1-1640
Alias Symbols: PEF1A, PEFLIN, ABP32
Protein Interaction Partner: FOXR2, KLHL12, CEP55, TFG, CALCOCO2, PDCD6, HGS, EWSR1, UBC, BAG3, DAZAP2, NR0B2, PDCD6IP, LAMTOR3, CUL3, H2AFX, ALG2,
Protein Size: 284

Molecular Weight: 31 kDa

Gene ID: 553115

NCBI Accession: NM_012392, NP_036524

UniProt: Q9UBV8