PEF1 antibody (Middle Region)

Catalog No. ABIN2787715

The Rabbit Polyclonal anti-PEF1 antibody (ABIN2787715) specifically detects PEF1 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for PEF1 antibody (Middle Region) (ABIN2787715)

Target: PEF1 (Penta-EF-Hand Domain Containing 1 (PEF1))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PEF1 Antibody

Binding Specificity: Middle Region

Sequence: WKFIQQWKNL FQQYDRDRSG SISYTELQQA LSQMGYNLSP QFTQLLVSRY

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 93%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against PEF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PEF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 284 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PEF1

Target: PEF1 (Penta-EF-Hand Domain Containing 1 (PEF1))

Alternative Name: PEF1

Background: PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1, MIM 114170), sorcin (SRI, MIM 182520), grancalcin (GCA, MIM 607030), and ALG2.PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1, MIM 114170), sorcin (SRI, MIM 182520), grancalcin (GCA, MIM 607030), and ALG2 (PDCD6, MIM 601057) (Kitaura et al., 2001 [PubMed 11278427]).[supplied by OMIM]. Sequence Note: removed 1 base from the 3' end that did not align to the reference genome assembly. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1640 AB026628.1 1-1640
Alias Symbols: PEF1A, PEFLIN, ABP32
Protein Interaction Partner: FOXR2, KLHL12, CEP55, TFG, CALCOCO2, PDCD6, HGS, EWSR1, UBC, BAG3, DAZAP2, NR0B2, PDCD6IP, LAMTOR3, CUL3, H2AFX, ALG2,
Protein Size: 284

Molecular Weight: 31 kDa

Gene ID: 553115

NCBI Accession: NM_012392, NP_036524

UniProt: Q9UBV8