BUB3 antibody (N-Term)

Catalog No. ABIN2787758

The Rabbit Polyclonal anti-BUB3 antibody has been validated for WB, IF and IHC. It is suitable to detect BUB3 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse and Rabbit.

Quick Overview for BUB3 antibody (N-Term) (ABIN2787758)

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-BUB3 Antibody

Binding Specificity: N-Term

Sequence: MTGSNEFKLN QPPEDGISSV KFSPNTSQFL LVSSWDTSVR LYDVPANSMR

Predicted Reactivity: Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against BUB3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BUB3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 326 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Alternative Name: BUB3

Background: BUB3 is required for kinetochore localization of BUB1.This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Alias Symbols: BUB3L, hBUB3
Protein Interaction Partner: BUB1B, BUB1, HUWE1, PSTPIP1, UBC, SUMO2, CDC20, NEDD8, SUMO1, RPA3, RPA2, RPA1, WBP4, CLIP1, EGFR, ZNF207, VCAM1, UBA1, THRA, RPL37A, ITGA4, FN1, NKAP, EXOC3, SUGT1, CPNE1, ANAPC4, SEPT2, TOP1, SMAD3, SMAD2, Mad2l1, COPS5, CUL3, SF3A2, ELAVL1, CDC27, HDGF
Protein Size: 326

Molecular Weight: 36 kDa

Gene ID: 9184

NCBI Accession: NM_001007793, NP_001007794

UniProt: A6NJ42

Pathways: Maintenance of Protein Location