UCHL3 antibody (N-Term)

Catalog No. ABIN2787791

This Rabbit Polyclonal antibody specifically detects UCHL3 in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio) and Goat.

Quick Overview for UCHL3 antibody (N-Term) (ABIN2787791)

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Reactivity: Human, Rat, Mouse, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-UCHL3 Antibody

Binding Specificity: N-Term

Purpose: UCHL3 Antibody - N-terminal region

Sequence: MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC

Predicted Reactivity: Cow: 100%, Goat: 82%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against UCHL3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UCHL3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58689-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for UCHL3

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Alternative Name: UCHL3

Background:

Background Information: UCHL3 is an ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8.

Gene Name: Ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)

Alternative Symbols: UCH-L3

Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L3

Molecular Weight: 26 kDa

Gene ID: 7347

NCBI Accession: NP_005993

UniProt: P15374

Pathways: Feeding Behaviour, Positive Regulation of fat Cell Differentiation