DAZ1 antibody (N-Term)

Catalog No. ABIN2787798

Product Details anti-DAZ1 Antibody

Target: DAZ1 (Deleted in Azoospermia 1 (DAZ1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Goat, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAZ1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MSAANPETPN STISREASTQ SSSAAASQGW VLPEGKIVPN TVFVGGIDAR

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 85%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against DAZ1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DAZ1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 579 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DAZ1 antibody (ABIN2787798)

Stouffs, Tournaye, Van der Elst, Haentjens, Liebaers, Lissens: "Do we need to search for gr/gr deletions in infertile men in a clinical setting?" in: Human reproduction (Oxford, England), Vol. 23, Issue 5, pp. 1193-9, (2008) (PubMed).

Target Details for DAZ1

Target: DAZ1 (Deleted in Azoospermia 1 (DAZ1))

Alternative Name: DAZ1 (DAZ1 Products)

Synonyms: DAZ1 antibody, DAZ4 antibody, DAZ antibody, SPGY antibody, deleted in azoospermia protein 1 antibody, deleted in azoospermia 1 antibody, LOC749905 antibody, DAZ1 antibody

Background: DAZ1 is a RNA-binding protein that plays an essential role in spermatogenesis. DAZ1 may act by binding to the 3'-UTR of mRNAs and regulating their translation.This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications, one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat, the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified, this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains three copies of the 10.8 kb repeat. However, no transcripts containing three copies of the RRM domain have been described, thus the RefSeq for this gene contains only two RRM domains. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DAZ, SPGY
Protein Interaction Partner: DZIP1L, BOLL, PUM2, DZIP1, QKI, DAZL, UBC, DAZ1, DZIP3, DAZAP2, DAZAP1, DYNLL1,
Protein Size: 579

Molecular Weight: 64 kDa

Gene ID: 1617

NCBI Accession: NM_004081, NP_004072

UniProt: Q9NQZ3