DAZ1 antibody (N-Term)

Catalog No. ABIN2787798

This Rabbit Polyclonal antibody specifically detects DAZ1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Horse, Dog, Goat, Pig and Rabbit.It has been mentioned in 1 publication

Quick Overview for DAZ1 antibody (N-Term) (ABIN2787798)

Target: DAZ1 (Deleted in Azoospermia 1 (DAZ1))

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Goat, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAZ1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DAZ1 Antibody

Binding Specificity: N-Term

Purpose: DAZ1 Antibody - N-terminal region

Sequence: MSAANPETPN STISREASTQ SSSAAASQGW VLPEGKIVPN TVFVGGIDAR

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 85%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against DAZ1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DAZ1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58711-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DAZ1 antibody (ABIN2787798)

Stouffs, Tournaye, Van der Elst, Haentjens, Liebaers, Lissens: "Do we need to search for gr/gr deletions in infertile men in a clinical setting?" in: Human reproduction (Oxford, England), Vol. 23, Issue 5, pp. 1193-9, (2008) (PubMed).

Target Details for DAZ1

Target: DAZ1 (Deleted in Azoospermia 1 (DAZ1))

Alternative Name: DAZ1

Background:

Background Information: DAZ1 is a RNA-binding protein that plays an essential role in spermatogenesis. DAZ1 may act by binding to the 3'-UTR of mRNAs and regulating their translation.This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications, one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat, the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified, this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains three copies of the 10.8 kb repeat. However, no transcripts containing three copies of the RRM domain have been described, thus the RefSeq for this gene contains only two RRM domains. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Deleted in azoospermia 1

Alternative Symbols: DAZ, SPGY

Protein Name: Deleted in azoospermia protein 1

Molecular Weight: 64kDa

Gene ID: 1617

NCBI Accession: NP_004072

UniProt: Q9NQZ3