Nestin antibody (Middle Region)

Catalog No. ABIN2787826

The Rabbit Polyclonal anti-Nestin antibody has been validated for WB and IHC. It is suitable to detect Nestin in samples from Human, Dog, Cow, Horse and Pig.

Quick Overview for Nestin antibody (Middle Region) (ABIN2787826)

Target: Nestin (NES)

Reactivity: Human, Dog, Cow, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Nestin Antibody

Binding Specificity: Middle Region

Sequence: LPDSTPLGFY LRSPTSPRWD PTGEQRPPPQ GETGKEGWDP AVLASEGLEA

Predicted Reactivity: Cow: 79%, Dog: 79%, Horse: 86%, Human: 100%, Pig: 86%

Characteristics: This is a rabbit polyclonal antibody against NES. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NES

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1621 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: NES

Background: Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neu
Alias Symbols: FLJ21841, Nbla00170
Protein Interaction Partner: SUMO3, UBC, SIRT7, CDK5R1, CDK5, SRRM1, CDK1,
Protein Size: 1621

Molecular Weight: 177 kDa

Gene ID: 10763

NCBI Accession: NM_006617, NP_006608

UniProt: P48681