Nestin antibody (Middle Region)

Catalog No. ABIN2787826

The Rabbit Polyclonal anti-Nestin antibody is suitable to detect Nestin in samples from Human, Mouse, Dog, Cow, Pig and Horse. It has been validated for IHC and WB.

Quick Overview for Nestin antibody (Middle Region) (ABIN2787826)

Target: Nestin (NES)

Reactivity: Human, Mouse, Dog, Cow, Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-Nestin Antibody

Binding Specificity: Middle Region

Purpose: NES Antibody - middle region

Sequence: LPDSTPLGFY LRSPTSPRWD PTGEQRPPPQ GETGKEGWDP AVLASEGLEA

Predicted Reactivity: Cow: 79%, Dog: 79%, Horse: 86%, Human: 100%, Pig: 86%

Characteristics: This is a rabbit polyclonal antibody against NES. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NES

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58779-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: NES

Background:

Background Information: Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neu

Gene Name: Nestin

Alternative Symbols: Nbla00170

Protein Name: Nestin

Molecular Weight: 177kDa

Gene ID: 10763

NCBI Accession: NP_006608

UniProt: P48681