AGO4 antibody (Middle Region)

Catalog No. ABIN2787830

The Rabbit Polyclonal anti-AGO4 antibody has been validated for WB. It is suitable to detect AGO4 in samples from Human, Mouse, Rat, Dog, Guinea Pig and Rabbit.

Quick Overview for AGO4 antibody (Middle Region) (ABIN2787830)

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AGO4 Antibody

Binding Specificity: Middle Region

Sequence: DGHPSRYCAT VRVQTSRQEI SQELLYSQEV IQDLTNMVRE LLIQFYKSTR

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF2C4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EIF2C4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 861 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for AGO4

Target: AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))

Alternative Name: EIF2C4

Background: This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic containing PAZ and PIWI domains, and it may play a role in short-interfering-RNA-mediated gene silencing. This gene i
Alias Symbols: AGO4, EIF2C4
Protein Interaction Partner: AGO3, APP, TNRC6C, TNRC6A, TNRC6B, UBC, RPL12P1, OTUD4P1, LOC390937, TUBB, AGO4, PGAM5, SNRPD2P1, RPS10P5, SLC25A22, WDR77, AGK, DNAJB11, AGO2, MYCBP, CCT5, RPL35, PTGES3, IGF2BP1, IPO8, PRMT5, TUBB4B, DNAJA2, SLC25A13, RPL23, SUCLA2, PABPC4, TUFM, SSBP1,
Protein Size: 861

Molecular Weight: 95 kDa

Gene ID: 192670

NCBI Accession: NM_017629, NP_060099

UniProt: Q9HCK5

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cellular Glucan Metabolic Process