ERK2 antibody (C-Term)

Catalog No. ABIN2787870

The Rabbit Polyclonal anti-ERK2 antibody has been validated for WB. It is suitable to detect ERK2 in samples from Human, Mouse, Rat, Dog, Cow, Rabbit, Horse, Zebrafish (Danio rerio), Guinea Pig and Goat.

Quick Overview for ERK2 antibody (C-Term) (ABIN2787870)

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Horse, Zebrafish (Danio rerio), Guinea Pig, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ERK2 Antibody

Binding Specificity: C-Term

Sequence: PYLEQYYDPS DEPIAEAPFK FDMELDDLPK EKLKELIFEE TARFQPGYRS

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MAPK1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MAPK1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 360 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: MAPK1

Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Alias Symbols: ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk
Protein Interaction Partner: Eif4ebp1, ATF2, RPS6KA3, RAF1, PTGDR, MAP2K6, MAP2K1, MAPK3, HDAC6, TP53, NEDD8, DGCR8, HMGB1, NAV1, PDCD6IP, CEP350, MAPK12, RPS6KA2, MAPK1, PDE4D, MBP, KRAS, SQSTM1, CUEDC2, GAB2, BRAF, RPS6KA1, MAP2K4, MAP2K2, ELK1, NOXA1, TIAL1, GADD45A, MAP3K10, SMAD
Protein Size: 360

Molecular Weight: 41 kDa

Gene ID: 5594

NCBI Accession: NM_138957, NP_620407

UniProt: P28482

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins