CD27 antibody (C-Term)

Catalog No. ABIN2787932

The Rabbit Polyclonal anti-CD27 antibody has been validated for WB. It is suitable to detect CD27 in samples from Human, Mouse, Rat, Pig, Cow, Dog, Horse, Guinea Pig and Rabbit.

Quick Overview for CD27 antibody (C-Term) (ABIN2787932)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD27 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD27 Antibody

Binding Specificity: C-Term

Sequence: LHQRRKYRSN KGESPVEPAE PCRYSCPREE EGSTIPIQED YRKPEPACSP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 75%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CD27. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CD27

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 260 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Alias Symbols: MGC20393, S152, T14, TNFRSF7, Tp55
Protein Interaction Partner: TRAF2, CD70, ELAVL1, UHRF2, SIVA1, TRAF5, TRAF3, TRAF1, CD5,
Protein Size: 260

Molecular Weight: 27 kDa

Gene ID: 939

NCBI Accession: NM_001242, NP_001233

UniProt: P26842

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints