MANF antibody (C-Term)

Catalog No. ABIN2788136

This Rabbit Polyclonal antibody specifically detects MANF in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for MANF antibody (C-Term) (ABIN2788136)

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MANF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MANF Antibody

Binding Specificity: C-Term

Sequence: EKICEKLKKK DSQICELKYD KQIDLSTVDL KKLRVKELKK ILDDWGETCK

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MANF. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MANF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 185 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MANF

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: MANF

Background: MANF is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of MANF gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of polymorphisms in the arginine-rich region, including a specific mutation that changes the previously numbered codon 50 from ATG to AGG, have been reported in a variety of solid tumors, however, these polymorphisms were later shown to exist in normal tissues and are thus not tumor-related.
Alias Symbols: ARMET, ARP, MGC142148, MGC142150
Protein Interaction Partner: UBC, MDM2,
Protein Size: 185

Molecular Weight: 21 kDa

Gene ID: 7873

NCBI Accession: NM_006010, NP_006001

UniProt: A8K878