HNMT antibody (N-Term)

Catalog No. ABIN2788140

The Rabbit Polyclonal anti-HNMT antibody is suitable to detect HNMT in samples from Human, Mouse, Rat, Guinea Pig, Cow, Dog, Horse, Pig and Rabbit. It has been validated for WB.

Quick Overview for HNMT antibody (N-Term) (ABIN2788140)

Target: HNMT (Histamine N-Methyltransferase (HNMT))

Reactivity: Human, Mouse, Rat, Guinea Pig, Cow, Dog, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNMT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HNMT Antibody

Binding Specificity: N-Term

Purpose: HNMT Antibody - N-terminal region

Sequence: PGIIGRIGDT KSEIKILSIG GGAGEIDLQI LSKVQAQYPG VCINNEVVEP

Predicted Reactivity: Cow: 92%, Dog: 92%, Guinea Pig: 77%, Horse: 92%, Human: 100%, Mouse: 77%, Pig: 92%, Rabbit: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against HNMT. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HNMT

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP59621-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HNMT

Target: HNMT (Histamine N-Methyltransferase (HNMT))

Alternative Name: HNMT

Background:

Background Information: In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.

Gene Name: Histamine N-methyltransferase

Alternative Symbols: HMT, MRT51, HNMT-S1, HNMT-S2

Protein Name: Histamine N-methyltransferase

Molecular Weight: 33kDa

Gene ID: 3176

NCBI Accession: NP_008826

UniProt: P50135