AP2A2 antibody (C-Term)

Catalog No. ABIN2788288

Product Details anti-AP2A2 Antibody

Target: AP2A2 (Adaptor-Related Protein Complex 2, alpha 2 Subunit (AP2A2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AP2A2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LAKLKKKKGP STVTDLEDTK RDRSVDVNGG PEPAPASTSA VSTPSPSADL

Predicted Reactivity: Dog: 79%, Guinea Pig: 79%, Human: 100%, Mouse: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against AP2A2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human AP2A2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 939 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for AP2A2

Target: AP2A2 (Adaptor-Related Protein Complex 2, alpha 2 Subunit (AP2A2))

Alternative Name: AP2A2 (AP2A2 Products)

Background: AP2A2 is a component of the adaptor protein complex 2 (AP-2). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif .
Alias Symbols: ADTAB, CLAPA2, HIP9, HYPJ, HIP-9
Protein Interaction Partner: PIP5K1C, UBC, ATP1A1, SUMO2, EGFR, RPA3, RPA2, RPA1, BIN1, PPP6R3, PAN2, SMAD1, FN1, DAB2, VCP, APP, AP2A1, SHC1, GRK5, EPS15, AP2M1, CLTC, GRB2, ARRB2, Rnf111, TERF2, DNAJC6, SNAP91, EPN1, AP2A2, NUMB, AP1S1, DNM1, AP2B1, AMPH, HTT, ATR, ATM, RALBP1,
Protein Size: 939

Molecular Weight: 104 kDa

Gene ID: 161

NCBI Accession: NM_012305, NP_036437

UniProt: O94973

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation, SARS-CoV-2 Protein Interactome