CYP24A1 antibody (C-Term)

Catalog No. ABIN2788353

The Rabbit Polyclonal anti-CYP24A1 antibody (ABIN2788353) specifically detects CYP24A1 in WB, IHC, IHC (p) and IHC (fp). The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Cow and Horse samples.

Quick Overview for CYP24A1 antibody (C-Term) (ABIN2788353)

Target: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP24A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-CYP24A1 Antibody

Binding Specificity: C-Term

Purpose: CYP24A1 Antibody - C-terminal region

Sequence: LNTKVWQDHT LAWDTIFKSV KACIDNRLEK YSQQPSADFL CDIYHQNRLS

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against CYP24A1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CYP24A1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP60147-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CYP24A1

Target: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Alternative Name: CYP24A1

Background:

Background Information: CYP24A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system.

Gene Name: Cytochrome P450, family 24, subfamily A, polypeptide 1

Alternative Symbols: CP24, HCAI, CYP24, HCINF1, P450-CC24

Protein Name: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

Molecular Weight: 55kDa

Gene ID: 1591

NCBI Accession: NP_000773

UniProt: Q07973

Pathways: Metabolism of Steroid Hormones and Vitamin D