AKR1C1 antibody (N-Term)

Catalog No. ABIN2788359

The Rabbit Polyclonal anti-AKR1C1 antibody (ABIN2788359) specifically detects AKR1C1 in WB and IHC. The antibody is reactive with Human, Rat, Mouse, Cow, Dog, Horse, Rabbit, Guinea Pig and Sheep samples.

Quick Overview for AKR1C1 antibody (N-Term) (ABIN2788359)

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Rabbit, Guinea Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKR1C1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-AKR1C1 Antibody

Binding Specificity: N-Term

Sequence: DSAHLYNNEE QVGLAIRSKI ADGSVKREDI FYTSKLWCNS HRPELVRPAL

Predicted Reactivity: Cow: 93%, Dog: 92%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against AKR1C1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AKR1C1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 323 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for AKR1C1

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Alternative Name: AKR1C1

Background: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.
Alias Symbols: 2-ALPHA-HSD, 20-ALPHA-HSD, C9, DD1, DDH, DDH1, H-37, HAKRC, MBAB, MGC8954, HBAB, DD1/DD2
Protein Interaction Partner: PTPN3, UBC, FN1, COMMD8, MAPK3, AKR1C1,
Protein Size: 323

Molecular Weight: 36 kDa

Gene ID: 1645

NCBI Accession: NM_001353, NP_001344

UniProt: Q04828

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process